| Summary: | Abstract In this study, shoots were regenerated from in vitro leaf tissues of ‘Prelude,’ a red raspberry known for its hardiness, vigorous growth, and early fruit ripening. Experiments were conducted to discover the effect of plant growth regulators (PGRs) on plant regeneration of ‘Prelude’ red raspberry. Four cytokinins, thidiazuron (TDZ), 6-benzylaminopurine (BA), zeatin, and kinetin, at various levels were analyzed for shoot regeneration when in vitro leaf tissues were cultured on woody plant medium (WPM). The maximum shoot regeneration rate was observed in the medium containing 0.5 μM TDZ with an average of 65.7% of the explants forming shoots. BA at 5.0 μM resulted in a shoot regeneration rate of 11.6%, and increasing the BA concentration slightly reduced the regeneration rate. Neither zeatin nor kinetin exhibited a significant effect on plant regeneration of ‘Prelude.’ In vitro shoots were dipped in a commercial rooting powder, TakeRoot®, and inserted into a potting medium for rooting and acclimatization. The average ex vitro rooting rate was 71.1%. Rooted plants were grown in the greenhouse. The development of an efficient plant regeneration system for ‘Prelude’ will facilitate the biotechnological improvement of raspberry and other Rubus species in the future.
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